Pneumococcal Hemolysin

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Diagnosis of pneumococcal pneumonia by enzyme-linked immunosorbent assay of antibodies to pneumococcal hemolysin (pneumolysin).

An enzyme-linked immunosorbent assay (ELISA) with a highly purified pneumolysin as the antigen was evaluated for serological diagnosis of pneumococcal pneumonia. One hundred four healthy controls were tested, and the specificity of the test was set to 95%. In samples from patients with bacteremic pneumococcal pneumonia, 82% (18 of 22) were positive, i.e., at least one serum sample had a titer a...

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Antigenic Streptococcal Hemolysin

1. Normal serum, used in cultures for preparation of the streptolysin, modifies the properties of the streptolysin, causing delayed hemolysis, increased filterability, resistance to oxidation or reduction, and absence of antigenicity. 1. Streptolysin prepared without serum is an active antigen. 3. Similar temperatures are required to destroy the antigenic activity of serum-free streptolysin and...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1942

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.43.5.607-627.1942